The formation of several by-products from the photodegradation of testosterone was previously observed under laboratory conditions. The objectives of the manuscript were to complete the identification of testosterone’s photoproducts and to develop an analytical method for the detection of testosterone as well as its three main photoproducts in natural sunlit surface waters. To accomplish these tasks, an efficient extraction method was developed based on solid-phase extraction, followed by the use of liquid chromatography coupled to tandem mass spectrometry, a selective and sensitive detection method. This analytical procedure has the capability of detecting target analytes in the ng/L range, with recoveries above 80 %. The methodology was successfully applied to the analysis of testosterone and its photoproducts in several surface waters.
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