ISSN: 2169-0111

Advancements in Genetic Engineering
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Gang (Mike) Zhang
Gang (Mike) Zhang Associate Executive Editor
Gang (Mike) Zhang

Division of Nephrology
Massachusetts General Hospital

I got my Ph. D. from Shandong Normal University and Chinese Academy of Sciences, China, majoring on the study of mouse pronuclear exchange and nuclear transfer in 2005. Then, I worked as a Postdoc Fellow at the Department of Cell and Systems Biology (2005-2007), and then moved to the Centre for Research in Neurodegenerative Diseases as a Research Technician II (2008-2012), University of Toronto, Canada. During this period, my research mainly focused on Parkinson disease, which included the differentiation of neural stem cells into dopamine neurons, and the molecular mechanism of Parkinson disease. Particularly, I developed a new method, designated as “Combinatorial Strategy” to clone different plasmid vectors with various clone sites, and also optimized the protocols of lentiviral transduction to establish stable transgene cell lines efficiently. Now, I am a Research Associate (Research Technician II) at Massachusetts General Hospital, Harvard University.

Research Interest

Gene Engineering, Transgene & Pathology: Lentiviral vector construction, production, titration, transduction, protein function analysis, various molecular cloning and PCR, site-directed mutagenesis, Western blotting, mechanism of Parkinson Disease, etc.

Cellular & Developmental Biology: Neural stem cell isolation, proliferation, differentiation, various tissue culture, immunostaining, brain tumor stem cell renewal and differentiation, establishment of transgene cell lines, etc.

Reproduction Biology: Mouse cloning, in vitro fertilization, in vivo and in vitro development of embryos, oocytes maturation, etc.


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